Composite

Part:BBa_K1392939

Designed by: Şeniz Yüksel   Group: iGEM14_METU_Turkey   (2014-10-07)


RBS + tetR-LVA

This part is a composite structure which contains a RBS side and and a tetracycline repressor from transposon Tn10. Through the RBS, the definition of the RBS efficiency could be observed. It is a coding region for the TetR protein with the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (Part:BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None